@article {640431,
	title = {Elongation factor-1alpha, a putative single-copy nuclear gene, has divergent sets of paralogs in an arachnid},
	journal = {Mol Phylogenet Evol},
	volume = {68},
	number = {3},
	year = {2013},
	note = {<p>Clouse, Ronald MSharma, Prashant PGiribet, GonzaloWheeler, Ward CengResearch Support, Non-U.S. Gov{\textquoteright}tResearch Support, U.S. Gov{\textquoteright}t, Non-P.H.S.2013/05/15 06:00Mol Phylogenet Evol. 2013 Sep;68(3):471-81. doi: 10.1016/j.ympev.2013.04.025. Epub 2013 May 10.</p>},
	month = {Sep},
	pages = {471-81},
	abstract = {<p>Identification of paralogy in candidate nuclear loci is an important prerequisite in phylogenetics and statistical phylogeography, but one that is often overlooked. One marker commonly assumed to be a single-copy gene and claimed to harbor great utility for inferring recent divergences is elongation factor-1alpha (EF-1alpha). To test this hypothesis, we systematically cloned EF-1alpha in three disjunct populations of the harvestman Metasiro americanus. Here we show that EF-1alpha has a large number of paralogs in this species. The paralogs do not evolve in a concerted manner, and the paralogs diverged prior to the population divergence. Moreover, the paralogs of M. americanus are not comparable to the highly divergent EF-1alpha paralogs found in bees and spiders, which are easily recognized and separated through the use of specific primers. We demonstrate statistically that our detection of paralogs cannot be attributed to amplification error. The presence of EF-1alpha paralogs in M. americanus prevents its use in statistical phylogeography, and the presence of out-paralogs argues against its use in phylogenetic inference among recently diverged clades. These data contradict the common assumption that EF-1alpha is for most or all taxa a single-copy gene, or that it has a small number of paralogs that are homogenized through gene conversion, unequal crossing over, or other processes.</p>},
	keywords = {*Gene Dosage, *Gene Duplication, Animals, Arachnida/*genetics, Cloning, Molecular, Evolution, Molecular, Gene Conversion, Genetic Variation, Molecular Sequence Data, Peptide Elongation Factor 1/*genetics, Phylogeny, Sequence Analysis, DNA},
	isbn = {1095-9513 (Electronic)1055-7903 (Linking)},
	author = {Clouse, R.M. and Sharma, P. P. and Giribet, G. and W.C. Wheeler}
}
