In Press
Worsaae, K., G. Giribet, and A. Martínez. In Press. “The role of progenesis in the diversification of the interstitial annelid lineage Psammodrilidae.” Invertebrate Systematics.
Kallal, R. J., R. Fernández, G. Giribet, and G. Hormiga. In Press. “. A phylotranscriptomic backbone of the orb-weaving spider family Araneidae (Arachnida, Araneae) supported by multiple methodological approaches. .” Molecular Phylogenetics and Evolution.
Giribet, G., R. Buckman-Young, C. Sampaio Costa, C.M. Baker, L. R. Benavides, M. G. Branstetter, S. R. Daniels, and R. Pinto-da-Rocha. In Press. “The "Peripatos" in Eurogondwana?-Lack of evidence that southeast Asian onychophorans walked through Europe. ”.
Giribet, G., and G.D. Edgecombe. Forthcoming. The Invertebrate Tree of Life.. March 2020: Princeton University Press.
Lemer, S., Bieler, R., and G. Giribet. 2019. “ Resolving the relationships of clams and cockles: dense transcriptome sampling drastically improves the bivalve tree of life .” Proceedings of the Royal Society B: Biological Sciences 286: 20182684. Abstract
Bivalvia has been the subject of extensive recent phylogenetic work to attempt resolving either the backbone of the bivalve tree using transcriptomic data, or the tips using morpho-anatomical data and up to five genetic markers. Yet the first approach lacked decisive taxon sampling and the second failed to resolve many interfamilial relationships, especially within the diverse clade Imparidentia. Here we combine dense taxon sampling with 108 deep-sequenced Illumina-based transcriptomes to provide resolution in nodes that required additional study. We designed specific data matrices to address the poorly resolved relationships within Imparidentia. Our results support the overall backbone of the bivalve tree, the monophyly of Bivalvia and all its main nodes, although the monophyly of Protobranchia remains less clear. Likewise, the inter-relationships of the six main bivalve clades were fully supported. Within Imparidentia, resolution increases when analysing Imparidentia-specific matrices. Lucinidae, Thyasiridae and Gastrochaenida represent three early branches. Gastrochaenida is sister group to all remaining imparidentians, which divide into six orders. Neoheterodontei is always fully supported, and consists of Sphaeriida, Myida and Venerida, with the latter now also containing Mactroidea, Ungulinoidea and Chamidae, a family particularly difficult to place in earlier work. Overall, our study, by using densely sampled transcriptomes, provides the best-resolved bivalve phylogeny to date.
Laumer, C.E.,, Fernández, R., Lemer, S., Combosch, D. J., Kocot, K., Andrade, S. C. S., Sterrer, W., M.V. Sørensen, and G. Giribet. 2019. “Revisiting metazoan phylogeny with genomic sampling of all phyla.” Proceedings of the Royal Society B: Biological Sciences 286: 20190831. Abstract
Proper biological interpretation of a phylogeny can sometimes hinge on the placement of key taxa-or fail when such key taxa are not sampled. In this light, we here present the first attempt to investigate (though not conclusively resolve) animal relationships using genome-scale data from all phyla. Results from the site-heterogeneous CAT + GTR model recapitulate many established major clades, and strongly confirm some recent discoveries, such as a monophyletic Lophophorata, and a sister group relationship between Gnathifera and Chaetognatha, raising continued questions on the nature of the spiralian ancestor. We also explore matrix construction with an eye towards testing specific relationships; this approach uniquely recovers support for Panarthropoda, and shows that Lophotrochozoa (a subclade of Spiralia) can be constructed in strongly conflicting ways using different taxon- and/or orthologue sets. Dayhoff-6 recoding sacrifices information, but can also reveal surprising outcomes, e.g. full support for a clade of Lophophorata and Entoprocta + Cycliophora, a clade of Placozoa + Cnidaria, and raising support for Ctenophora as sister group to the remaining Metazoa, in a manner dependent on the gene and/or taxon sampling of the matrix in question. Future work should test the hypothesis that the few remaining uncertainties in animal phylogeny might reflect violations of the various stationarity assumptions used in contemporary inference methods.
Giribet, G., and G.D. Edgecombe. 2019. “The phylogeny and evolutionary history of arthropods.” Current Biology 29: R592-R602. Abstract
Arthropods are the most diverse animal phylum, and their phylogenetic relationships have been debated for centuries. With the advent of molecular phylogenetics, arthropods were found to be monophyletic and placed within a clade of molting animals, the ecdysozoans, with nematodes and six other phyla. Molecular phylogenetics also provided a new framework for relationships between the major arthropod groups, such as the clade Pancrustacea, which comprises insects and crustaceans. Phylogenomics based on second-generation genomics and transcriptomics has further resolved puzzles such as the exact position of myriapods or the closest crustacean relatives of hexapods. It is now broadly recognized that extant arthropods are split into chelicerates and mandibulates, and relationships within the two mandibulate clades (myriapods and pancrustaceans) are stabilizing. Notably, the phylogeny of insects is now understood with considerable confidence, whereas relationships among chelicerate orders remain poorly resolved. The evolutionary history of arthropods is illuminated by a rich record of fossils, often with exquisite preservation, but current analyses conflict over whether certain fossil groups are stem- or crown-group arthropods. Molecular time-trees calibrated with fossils estimate the origins of arthropods to be in the Ediacaran, while most other deep nodes date to the Cambrian. The earliest stem-group arthropods were lobopodians, worm-like animals with annulated appendages. Confidently placing some key extinct clades on the arthropod tree of life may require less ambiguous interpretation of fossil structures and better integration of morphological data into the phylogeny.
Giribet, G., and C.M. Baker. 2019. “Further discussion on the Eocene drowning of New Caledonia: discordances from the point of view of zoology.” Journal of Biogeography 46: 1912-1918. Abstract
We discuss the fauna of New Caledonia in the context of the prolonged submergence of Grande Terre until its re‐emergence around 37 million years ago and whether the resulting fauna can be entirely explained by over‐water dispersal. The current literature discussing the predominant neoendemism in New Caledonia is reviewed, questioning some of the discourse about how the fact that most animal and plant lineages are neoendemics should weigh in to disregard the fewer cases of paleoendemism (clades that have persisted and diversified in New Caledonia for over 37 million years). We argue that many of the examples used in the literature, selected for other purposes, were not chosen to test this particular hypothesis, but several old lineages of non‐vagile animals show that a non‐trivial number of clades have a history that predates the supposed emergence of New Caledonia. We conclude by posing the question of how much additional evidence should be needed to demonstrate a discordance between the geological history of the archipelago and the evolutionary history of its biota.
Derkarabetian, S, L. R. Benavides, and G. Giribet. 2019. “Sequence capture phylogenomics of historical ethanol-preserved museum specimens: unlocking the rest of the vault.” Molecular Ecology Resources 00: 1-14. Abstract
Natural history collections play a crucial role in biodiversity research, and museum specimens are increasingly being incorporated into modern genetics‐based studies. Sequence capture methods have proven incredibly useful for phylogenomics, providing the additional ability to sequence historical museum specimens with highly degraded DNA, which until recently have been deemed less valuable for genetic work. The successful sequencing of ultraconserved elements (UCEs) from historical museum specimens has been demonstrated on multiple tissue types including dried bird skins, formalin‐fixed squamates and pinned insects. However, no study has thoroughly demonstrated this approach for historical ethanol‐preserved museum specimens. Alongside sequencing of “fresh” specimens preserved in >95% ethanol and stored at −80°C, we used extraction techniques specifically designed for degraded DNA coupled with sequence capture protocols to sequence UCEs from historical museum specimens preserved in 70%–80% ethanol and stored at room temperature, the standard for such ethanol‐preserved museum collections. Across 35 fresh and 15 historical museum samples of the arachnid order Opiliones, an average of 345 UCE loci were included in phylogenomic matrices, with museum samples ranging from six to 495 loci. We successfully demonstrate the inclusion of historical ethanol‐preserved museum specimens in modern sequence capture phylogenomic studies, show a high frequency of variant bases at the species and population levels, and from off‐target reads successfully recover multiple loci traditionally sequenced in multilocus studies including mitochondrial loci and nuclear rRNA loci. The methods detailed in this study will allow researchers to potentially acquire genetic data from millions of ethanol‐preserved museum specimens held in collections worldwide.
Cunha, T. J., and G. Giribet. 2019. “A congruent topology for deep gastropod relationships.” Proceedings of the Royal Society B: Biological Sciences 286: 20182776. Abstract
Gastropod molluscs are among the most diverse and abundant animals in the oceans, and are successful colonizers of terrestrial and freshwater environments. Past phylogenetic efforts to resolve gastropod relationships resulted in a range of conflicting hypotheses. Here, we use phylogenomics to address deep relationships between the five major gastropod lineages—Caenogastropoda, Heterobranchia, Neritimorpha, Patellogastropoda and Vetigastropoda—and provide one congruent and well-supported topology. We substantially expand taxon sampling for outgroups and for previously underrepresented gastropod lineages, presenting new transcriptomes for neritimorphs and patellogastropods. We conduct analyses under maximum-likelihood, Bayesian inference and a coalescent-based approach, accounting for the most pervasive sources of systematic errors in large datasets: compositional heterogeneity, site heterogeneity, heterotachy, variation in evolutionary rates among genes, matrix completeness, outgroup choice and gene tree conflict. We find that vetigastropods and patellogastropods are sister taxa, and that neritimorphs are the sister group to caenogastropods and heterobranchs. We name these two major unranked clades Psilogastropoda and Angiogastropoda, respectively. We additionally provide the first genomic-scale data for internal relationships of neritimorphs and patellogastropods. Our results highlight the need for reinterpreting the evolution of morphological and developmental characters in gastropods, especially for inferring their ancestral states.
Benavides, L. R., G. Hormiga, and G. Giribet. 2019. “ Phylogeny, evolution and systematic revision of the mite harvestman family Neogoveidae (Opiliones Cyphophthalmi).” Invertebrate Systematics 33: 101-180. Abstract
Mite harvestmen of the family Neogoveidae have a tropical trans-Atlantic distribution with representatives in equatorial West Africa and the Neotropics, specifically in the south-east region of the USA and in northern South America, being conspicuously absent from Central America. We provide a comprehensive molecular phylogeny of the family including representatives of all genera but the monotypic Tucanogovea Karaman, 2013, and new information on the type species described by Jochen Martens in 1969 that were unavailable for molecular study until now: Brasiliogovea microphaga, Metagovea oviformis and ‘? Gen. enigmaticus’. Additionally, we revisit the somatic and male genitalic morphology of representatives of all genera by means of scanning electron microscopy and confocal laser scanning microscopy, and describe the new genera Leggogovia Benavides & Giribet, gen. nov., Microgovia Benavides, Hormiga & Giribet, gen. nov., Waiwaigovia Benavides, Hormiga & Giribet, gen. nov. and 13 new species: Brasiliogovea aphantostylus Benavides, Hormiga & Giribet, sp. nov., Brasiliogovea microstylus Benavides, Hormiga & Giribet, sp. nov., Brasiliogovea yacambuensis Benavides, Hormiga & Giribet, sp. nov., Metagovea matapi Benavides, Hormiga & Giribet, sp. nov., Metagovea planada Benavides, Hormiga & Giribet, sp. nov., Microgovia chenepau Benavides, Hormiga & Giribet, sp. nov., Neogovea branstetteri Benavides, Hormiga & Giribet, sp. nov., Neogovea enigmatica Martens, sp. nov., Neogovea matawai Benavides, Hormiga & Giribet, sp. nov., Parogovia montealensis Benavides & Giribet, sp. nov., Parogovia prietoi Benavides & Giribet, sp. nov., Parogovia putnami Benavides & Giribet, sp. nov. and Waiwaigovia schultzi Benavides, Hormiga & Giribet, sp. nov. Phylogenetic analyses based on maximum likelihood, parsimony and Bayesian inference support the monophyly of Neogoveidae and a sister group relationship of Neogoveidae + Ogoveidae with Troglosironidae (a clade named Sternophthalmi). Relationships among neogoveid genera are largely congruent between methods as follows: ((Leggogovia gen. nov., Metasiro), (Parogovia, ((Canga, Microgovia gen. nov.), ((Brasiliogovea, Neogovea), (Huitaca, (Waiwaigovia gen. nov., Metagovea)))))). In light of our results, the following taxonomic changes are proposed: Metagovea oviformis Martens, 1969 is transferred to Microgovia, gen. nov.; Parogovia pabsgarnoni Legg, 1990 is transferred to Leggogovia, gen. nov.; ‘? Gen. enigmaticus Martens, 1969’ is an invalid name according to the ICZN; the corresponding taxon is redescribed and formally named as Neogovea enigmatica Martens, sp. nov.
Benavides, L. R., J. G. Cosgrove, M. S. Harvey, and G. Giribet. 2019. “Phylogenomic interrogation resolves the backbone of the Pseudoscorpiones Tree of Life.” Molecular Phylogenetics and Evolution 139: 106509. Abstract
Pseudoscorpiones, with nearly 3700 described species, are an ancient and globally distributed group of arachnids with a fossil record dating back to the Middle Devonian. Previous attempts to reconstruct their phylogenetic history have used morphology or a few amplicons, mostly of rRNAs and mitochondrial genes, which have not been able to completely resolve family-level relationships nor the earliest nodes in the pseudoscorpion tree—those which are most informative about the origins of key characters like venoms and silk. Here we undertake a phylogenetic approach using 41 pseudoscorpion transcriptomes and a series of analyses that account for many of the common pitfalls faced in large phylogenomic analyses. All analyses, using concatenation methods and coalescent approaches, supported monophyly of Iocheirata (the venomous pseudoscorpions), which diversified mostly during the Mesozoic, but paraphyly of Epiocheirata, with a sister group relationship of Feaelloidea to Iocheirata, with Chthonioidea as their sister group. These three main lineages were established during the mid-to-late Paleozoic. Our phylogenetic scheme is consistent with the prior hypothesis that the lack of venom in Pseudoscorpiones is plesiomorphic and not a synapomorphy of Epiocheirata. Based on the results of this study, a new classification is proposed for Pseudoscorpiones including the following new nomenclatural and taxonomic acts: the new suborders Palaeosphyronida Harvey and Atoposphyronida Harvey for Dracochelidae and Feaelloidea, respectively; the newly recognized superfamily Garypinoidea for Garypinidae and Larcidae; the revised rank for Lechytiidae and Tridenchthoniidae, which are regarded as subfamilies of Chthoniidae; the revised rank for Tridenchthoniini and Verrucadithini which are regarded as tribes of Tridenchthoniinae; and the elevation of Hesperolpiinae as a distinct family, Hesperolpiidae.
Sharma, P. P., C. Baker, J. G. Cosgrove, J. Johnson, J. Oberski, R. Raven, M. Harvey, S. Boyer, and G. Giribet. 2018. “A revised dated phylogeny of scorpions: Phylogenomic support for ancient divergence of the temperate Gondwanan family Bothriuridae. .” Molecular Phylogenetics and Evolution 122: 37-45. Abstract
The scorpion family Bothriuridae occupies a subset of landmasses formerly constituting East and West temperate Gondwana, but its relationship to other scorpion families is in question. Whereas morphological data have strongly supported a sister group relationship of Bothriuridae and the superfamily Scorpionoidea, a recent phylogenomic analysis recovered a basal placement of bothriurids within Iurida, albeit sampling only a single exemplar. Here we reexamined the phylogenetic placement of the family Bothriuridae, sampling six bothriurid exemplars representing both East and West Gondwana, using transcriptomic data. Our results demonstrate that the sister group relationship of Bothriuridae to the clade ("Chactoidea" + Scorpionoidea) is supported by the inclusion of additional bothriurid taxa, and that this placement is insensitive to matrix completeness or partitioning by evolutionary rate. We also estimated divergence times within the order Scorpiones using multiple fossil calibrations, to infer whether the family Bothriuridae is sufficiently old to be characterized as a true Gondwanan lineage. We show that scorpions underwent ancient diversification between the Devonian and early Carboniferous. The age interval of the bothriurids sampled (a derived group that excludes exemplars from South Africa) spans the timing of breakup of temperate Gondwana.
Huang, D., G. Hormiga, F. Xia, C. Cai, Z. Yin, Y. Su, and G. Giribet. 2018. “Origin of spiders and their spinning organs illuminated by mid-Cretaceous amber fossils. .” Nature Ecology & Evolution 2: 623-627.
A recent paper in BMC Biology has resolved the family relationships of sea squirts, one of our closest invertebrate relatives, by using a large phylogenomic data set derived from available genomes and newly generated transcriptomes. The work confirms previous ideas that ascidians (the sea squirts) are not monophyletic, as they include some pelagic jelly-like relatives, and proposes a chronogram for a group that has been difficult to resolve due to their accelerated genome evolution.
Giribet, G. 2018. “Current views on chelicerate phylogeny—A tribute to Peter Weygoldt.” Zoologischer Anzeiger 273,: 7-13.
Gainett, G., P. P. Sharma, G. Giribet, and R. H. Willemart. 2018. “Putative adhesive setae on the walking legs of the Paleotropical harvestman Metibalonius sp. (Arachnida: Opiliones: Podoctidae). .” Journal of Arachnology 46: 62-68. Abstract
We provide a first scanning electron microscopy examination of the Paleotropical harvestman family Podoctidae (Opiliones: Laniatores), focusing on the distitarsus of the legs of Metibalonius sp. Distitarsi I and II are mostly equipped with olfactory sensilla chaetica with wall pores, while those of legs III and IV have gustatory sensilla chaetica with a tip pore, ventral trichomes with ovate tips (non-sensory) and a type of spatulate seta. Spatulate setae are present in adults of both sexes, with no apparent sexual dimorphism, but they are absent in the nymph. Seven of these setae are inserted on the frontal surface of the last tarsomere of legs III and IV, with the tips oriented ventrally. Each seta has an s-shaped socketed shaft, which terminates distally in a spatula-shaped structure. The distribution of spatulate setae, restricted to legs III and IV (walking legs), the position on the distitarsi, and the typical spatulate shape suggest an adhesive function for these structures. Morphology and position suggest that the socketed spatulate setae of Metibalonius sp. and the previously reported scopular spatulate setae of other harvestmen constitute two distinct types of adhesive structures, highlighting the diversity of adhesive structures in Laniatores. Future investigations about the natural history of this species and internal morphology of spatulate setae are necessary to test further functional hypotheses and to determine their behavioral role.
Fernández, R., R. J. Kallal, D. Dimitrov, J. A. Ballesteros, M. A. Arnedo, G. Giribet, and G. Hormiga. 2018. “Phylogenomics, diversification dynamics and comparative transcriptomics across the Spider Tree of Life. .” Current Biology.
The interrelationships of the four classes of Myriapoda have been an unresolved question in arthropod phylogenetics and an example of conflict between morphology and molecules. Morphology and development provide compelling support for Diplopoda (millipedes) and Pauropoda being closest relatives, and moderate support for Symphyla being more closely related to the diplopod-pauropod group than any of them are to Chilopoda (centipedes). In contrast, several molecular datasets have contradicted the Diplopoda–Pauropoda grouping (named Dignatha), often recovering a Symphyla–Pauropoda group (named Edafopoda). Here we present the first transcriptomic data including a pauropod and both families of symphylans, allowing myriapod interrelationships to be inferred from phylogenomic data from representatives of all main lineages. Phylogenomic analyses consistently recovered Dignatha with strong support. Taxon removal experiments identified outgroup choice as a critical factor affecting myriapod interrelationships. Diversification of millipedes in the Ordovician and centipedes in the Silurian closely approximates fossil evidence whereas the deeper nodes of the myriapod tree date to various depths in the Cambrian-Early Ordovician, roughly coinciding with recent estimates of terrestrialisation in other arthropod lineages, including hexapods and arachnids.
Voolstra, C., M. Adamski, S. Agrawal, A. Antunes, M. Aranda, Baxevanis, A., M. Blaxter, T. Bosch, H. Bracken-Grissom, and J. Coddington. 2017. “Advancing genomics through the Global Invertebrate Genomics Alliance (GIGA.” Invertebrate Systematics 31: 1-7. Abstract
The Global Invertebrate Genomics Alliance (GIGA), a collaborative network of diverse scientists, marked its second anniversary with a workshop in Munich, Germany in 2015, where international attendees focused on discussing current progress, milestones and bioinformatics resources. The community determined the recruitment and training of talented researchers as one of the most pressing future needs and identified opportunities for network funding. GIGA also promotes future research efforts to prioritise taxonomic diversity and create new synergies. Here, we announce the generation of a central and simple data repository portal with a wide coverage of available sequence data, via the compagen platform, in parallel with more focused and specialised organism databases to globally advance invertebrate genomics. This article serves the objectives of GIGA by disseminating current progress and future prospects in the science of invertebrate genomics with the aim of promotion and facilitation of interdisciplinary and international research.